Mat Titers Leptospirosis

For the reading of mat titers it is highly recommended to use a darkfield microscope with a high contrast between the leptospires and the dark background.

Mat titers leptospirosis.

Results are expressed as the reciprocal of the highest dilution where agglutination is observed. Horses that abort due to leptospirosis exposure tend to have very high mat titers. If the level of antibodies called a titer is high enough or can be shown to be rising over time then infection is confirmed. In leptospirosis antibodies can be demonstrated in the serum by mat.

Testing may include culture and or serology. A severe form which includes jaundice kidney failure and bleeding is called weil disease. Titers against serovar autumnalis are available in our expanded panel test code 95066 or as an add on test code 95054 by request. Human leptospirosis is an acute febrile illness that presents with many manifestations and is found worldwide.

Does the mat always work. A series of cultures of different leptospires are incubated with serial dilutions of serum. What is the mat test for leptospirosis. As with other serology assays vaccination complicates the interpretation of titers.

Major disadvantages of microscopic agglutination test mat. The mat is an excellent test but it. Sera are screened at a 1 100 dilution and those showing agglutination are then serially diluted further to determine a titer endpoint. A single elevated mat titer in a patient with a compatible febrile illness and suspected exposure suggests acute leptospirosis.

Lepto mat result interpretation the mat is a qualitative and quantitative test having high diagnostic specificity and relatively low sensitivity. The mat or microscopic agglutination test detects the presence of antibodies against leptospira in a dog s blood. Laboratory studies can confirm the diagnosis and determine the extent of organ involvement. Detection of antibodies using the microscopic agglutination test mat has been the most common diagnostic method used for the diagnosis of canine leptospirosis 10 vaccination with commercially available leptospirosis vaccines will produce detectable mat titers 10 see algorithm 2 for more information on mat results.

For all 108 patients leptospirosis was diagnosed on the basis of a fourfold or greater increase in titer by mat seroconversion and all but 1 of 245 controls were mat negative titers 1 100. For all species of animals when chronic leptospirosis is suspected abortion anterior uveitis hepatitis and nephritis etc it is not necessary to collect a convalescent serum sample. In the endemic regions there may be a substantial proportion of the population with elevated titres of mat. Titers of greater than 100 are usually considered significant although these may be due to vaccination.